分类: 计算机科学 >> 计算机科学的集成理论 提交时间: 2022-11-18 合作期刊: 《数据智能(英文)》
摘要: With the rapid growth of the linked data on the Web, the quality assessment of the RDF data set becomes particularly important, especially for the quality and accessibility of the linked data. In most cases, RDF data sets are shared online, leading to a high maintenance cost for the quality assessment. This also potentially pollutes Internet data. Recently blockchain technology has shown the potential in many applications. Using the blockchain storage quality assessment results can reduce the centralization of the authority, and the quality assessment results have characteristics such as non-tampering. To this end, we propose an RDF data quality assessment model in a decentralized environment, pointing out a new dimension of RDF data quality. We use the blockchain to record the data quality assessment results and design a detailed update strategy for the quality assessment results. We have implemented a system DCQA to test and verify the feasibility of the quality assessment model. The proposed method can provide users with better cost-effective results when knowledge is independently protected.
分类: 生物学 >> 生物物理学 提交时间: 2016-05-12
摘要: RNA virus infection is recognized by the RIG-I family of receptors that activate the mitochondrial adaptor MAVS, leading to the clearance of viruses. Antiviral signalling activation requires strict modulation to avoid damage to the host from exacerbated inflammation. Insulin receptor tyrosine kinase substrate (IRTKS) participates in actin bundling and insulin signalling and its deficiency causes insulin resistance. However, whether IRTKS is involved in the regulation of innate immunity remains elusive. Here we show that IRTKS deficiency causes enhanced innate immune responses against RNA viruses. IRTKS-mediated suppression of antiviral responses depends on the RIG-I-MAVS signalling pathway. IRTKS recruits the E2 ligase Ubc9 to sumoylate PCBP2 in the nucleus, which causes its cytoplasmic translocation during viral infection. The sumoylated PCBP2 associates with MAVS to initiate its degradation, leading to downregulation of antiviral responses. Thus, IRTKS functions as a negative modulator of excessive inflammation.
分类: 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间: 2016-05-12
摘要: Sulfolobus synthesizes large amounts of small chromatin proteins Cren7 and Sul7d. The two proteins share overall structural similarity, but differ distinctly in the DNA-binding region between beta 3- and beta 4-strands. While Sul7d possesses a hinge of two amino acid residues, Cren7 contains a flexible seven-residue loop (loop beta 3-beta 4) in the region. Here, we report the role of loop beta 3-beta 4 in the interaction of Cren7 with duplex DNA. We show that all residues with a large side chain on the loop, i.e., Pro30, Lys31, Arg33 and Lys34, contributed significantly to the binding of Cren7 to DNA. The three basic amino acids affected the ability of Cren7 to constrain negative DNA supercoils in a residue number-dependent manner. The crystal structure of a complex between a mutant Cren7 protein (GR) with loop beta 3-beta 4 replaced by two residues (Gly and Arg) to mimic the hinge at the corresponding position in Sul7d and an 8-bp dsDNA has been determined. Structural comparison between the GR-DNA and Cren7-DNA complexes shows that GR resembles Sul7d more than Cren7 in DNA-binding size and in the effect on the width of the major groove of DNA and the pattern of DNA bending. However, GR induces smaller DNA curvature than Sul7d. Our results suggest that Cren7 and Sul7d package chromosomal DNA in a slightly different fashion, presumably permitting different chromosomal accessibility by proteins functioning in DNA transactions.
分类: 天文学 >> 天文学 提交时间: 2024-02-28 合作期刊: 《Research in Astronomy and Astrophysics》
摘要: The scattered stray light of a coronagraph is a type of stray light that is generated by the objective lens as its surface defects are irradiated by sunlight. The defects mainly include dust and blemishes on the lens surface, microroughness of the lens surface, and impurity and inhomogeneity of the glass. Unlike the other types of relatively stable defects introduced when the objective lens is being manufactured, the scattered stray light caused by dusts on the lens surface is difficult to quantify accurately due to the disorder and randomness of the dust accumulation. The contribution of this type of stray light to the overall stray light level is difficult to determine through simulations and experiments. This can result in continuous deterioration of the stray light level of a coronagraph and thus affect the observation capabilities of the instrument. To solve this issue, through analyzing the forming mechanism of scattered stray light and ghost image generated by the inner-occulted coronagraph, we propose a novel method to monitor the scattered stray light from dusts by utilizing different stray light correlation coefficients. In this method, we first simulate and measure the level of stray light from the ghost image of the objective lens, and then determine the flux ratio of scattered light and ghost image on the conjugate plane. Although the flux ratio varies with the accumulation of dusts on the lens surface, it remains constant on the image plane. Therefore, the level of dust scattering light on the image plane can be obtained by using this ratio together with the level of ghost image stray light. The accuracy of this method has been validated in a laboratory by applying the objective lens with numerous surface cleanliness levels.
分类: 生物学 >> 生物物理学 >> 神经科学 提交时间: 2016-05-11
摘要: All neurodegenerative diseases are associated with oxidative stress-induced neuronal death. Forkhead box O3a (FOXO3a) is a key transcription factor involved in neuronal apoptosis. However, how FOXO3a forms complexes and functions in oxidative stress processing remains largely unknown. In the present study, we show that histone deacetylase 2 (HDAC2) forms a physical complex with FOXO3a, which plays an important role in FOXO3a-dependent gene transcription and oxidative stress-induced mouse cerebellar granule neuron (CGN) apoptosis. Interestingly, we also found that HDAC2 became selectively enriched in the promoter region of the p21 gene, but not those of other target genes, and inhibited FOXO3a-mediated p21 transcription. Furthermore, we found that oxidative stress reduced the interaction between FOXO3a and HDAC2, leading to an increased histone H4K16 acetylation level in the p21 promoter region and upregulated p21 expression in a manner independent of p53 or E2F1. Phosphorylation of HDAC2 at Ser 394 is important for the HDAC2-FOXO3a interaction, and we found that cerebral ischemia/reperfusion reduced phosphorylation of HDAC2 at Ser 394 and mitigated the HDAC2-FOXO3a interaction in mouse brain tissue. Our study reveals the novel regulation of FOXO3a-mediated selective gene transcription via epigenetic modification in the process of oxidative stress-induced cell death, which could be exploited therapeutically.
分类: 生物学 >> 生物物理学 >> 细胞生物学 提交时间: 2016-05-12
摘要: Specific recognition of centromere-specific histone variant CENP-A-containing chromatin by CENP-N is an essential process in the assembly of the kinetochore complex at centromeres prior to mammalian cell division. However, the mechanisms of CENP-N recruitment to centromeres/kinetochores remain unknown. Here, we show that a CENP-A-specific RG loop (Arg80/Gly81) plays an essential and dual regulatory role in this process. The RG loop assists the formation of a compact "ladder-like" structure of CENP-A chromatin, concealing the loop and thus impairing its role in recruiting CENP-N. Upon G1/S-phase transition, however, centromeric chromatin switches from the compact to an open state, enabling the now exposed RG loop to recruit CENP-N prior to cell division. Our results provide the first insights into the mechanisms by which the recruitment of CENP-N is regulated by the structural transitions between compaction and relaxation of centromeric chromatin during the cell cycle.